Peptide Delivery to Cell Cultures Using Intelligent Polymers

使用智能聚合物将肽递送至细胞培养物

• 批准号: 9707652
• 负责人: Kimberly Brown
• 金额: $ 5.94万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707652&HistoricalAwards=false
• 关键词: Peptide; Delivery; Cell; Cultures; Using

9707652 Brown This is a promising new investigator in the field of biomaterials and tissue engineering. She has great potential to become a fine scientist. Funding of this proposal is expected to advance the PI's career and meet the goals of this program. The proposed research investigates novel polymers that swell and collapse in response to electrical stimuli. These materials may be useful for modulated drug delivery. The PI has already constructed a prototype of the electrorelease chamber used in these studies and has an appropriate background to conduct the proposed studies. In addition to potential drug delivery applications, the proposed technology may be useful for in vitro evaluation of cellular responses to various substances. As technical notes, LDH is a crude measure of cytotoxicity and biocompatibility, as it detects rupture of cell membranes. Changes in insulin production, cell metabolism, etc. will be observed long before membrane rupture. Inclusion of a diagram of the electrorelease chamber would have been helpful, in that the photograph provided was not very clear. ***

9707652 Brown这是生物材料和组织工程领域中有希望的新研究者。 她有很大的潜力成为一名优秀的科学家。 预计该提案的资金将推进PI的职业生涯并实现该计划的目标。 拟议的研究调查了对电刺激的响应膨胀和崩溃的新型聚合物。 这些材料可能可用于调制药物输送。 PI已经构建了这些研究中使用的电子释放室的原型，并且具有适当的背景来进行拟议的研究。 除了潜在的药物递送应用外，该提出的技术可能对体外评估细胞对各种物质的反应。 正如技术指出的那样，LDH是细胞毒性和生物相容性的粗略度量，因为它检测到细胞膜破裂。 在膜破裂之前，将观察到胰岛素产生，细胞代谢等的变化。 包括电子发行室的图表将很有帮助，因为所提供的照片并不清楚。 ***