Two-Stage Fermentation of Whey Permeate for Biodegradable Deicer Production

用于可生物降解除冰剂生产的乳清渗透物的两阶段发酵

• 批准号: 9613273
• 负责人: Alexander Mathews
• 金额: $ 24.29万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9613273&HistoricalAwards=false
• 关键词: Two; Stage; Fermentation; Whey; Permeate

9613273 Mathews Biomass wastes such as liquid whey effluents from the dairy industry are a burden on the environment due to their high biochemical oxygen demand (BOD). Treatment of these wastes to meet environmental standards is both capital and energy intensive. This proposal examines the use of whey permeate in the production of a road deicer substitute for sodium chloride, since currently used deicers cause extensive corrosion-related damage to the highway infrastructure and environmental damage to water supplies and soils. The proposed research will examine the use of biomass wastes in the production of substitute deicers, calcium magnesium acetate (CMA) and calcium magnesium propionate (CMP), via a two-stage bacterial fermentation process. In addition, a mathematical model will be to describe continuous cell- recycle fermentation in coculture systems. Growth kinetics studies will be conducted using the bacteria Propionibacterium acidipropionici and Lactobacillus plantarum in monoculture and coculture systems. The effect of substrate concentration, pH and other parameters on growth and organic acid productivity will be determined. A synergistic growth kinetics model that includes substrate and product inhibition kinetics will be developed, and kinetic constants will be obtained from the data. Continuous flow cell-recycle fermentation studies will be conducted for coculture and two-stage fermentation systems to determine acid productivity and product concentrations. A model will be developed to describe continuous flow cell- recycle fermentation for single and coculture systems, and the model will be verified with the experimental data. This research will develop data and basic knowledge required in the design of an innovative fermentation system for the conversion of whey effluents to useful chemicals. The methodology and approach developed can be utilized in processes to convert a variety of inexpensive sugar substrates and biomass hydrolysat es into low-cost biodegradable deicers.

9613273 Mathews生物量废物（例如奶牛产业的液体乳清废物）由于其高生物化学氧需求（BOD）而对环境负担。这些废物以满足环境标准的处理是资本和能源密集型的。 该提案研究了乳清渗透在生产氯化钠的道路脱水剂替代品中的使用，因为目前使用的脱水剂对高速公路基础设施造成了广泛的与腐蚀相关的损害，以及对水供应和土壤的环境损害。拟议的研究将通过两阶段的细菌发酵过程来研究生物量废物在替代脱水剂，乙酸钙（CMA）和丙酸钙（CMP）的产生中的使用。 另外，数学模型将是描述共培养系统中连续的细胞回收发酵。 生长动力学研究将使用单一培养和共培养系统中的酸性细菌和乳杆菌pr杆菌进行。 将确定底物浓度，pH和其他参数对生长和有机酸生产率的影响。 将开发包括底物和产品抑制动力学的协同生长动力学模型，并将从数据中获得动力学常数。 将针对共培养和两阶段发酵系统进行连续的流细胞生物发酵研究，以确定酸生产力和产物浓度。 将开发一个模型来描述单个和共培养系统的连续流细胞回收发酵，并将通过实验数据验证该模型。 这项研究将在设计创新的发酵系统设计中开发数据和基本知识，以将乳清废物转化为有用的化学物质。 开发的方法和方法可用于将各种廉价的糖基质和生物质水解质量转化为低成本生物降解的脱水剂。