The Cell Biology and Genetics of the Maize Meiotic Kinetochore

玉米减数分裂着丝粒的细胞生物学和遗传学

• 批准号: 9505670
• 负责人: R Kelly Dawe
• 金额: $ 3.5万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9505670&HistoricalAwards=false
• 关键词: Cell; Biology; Genetics; Maize; Meiotic

; R o o t E n t r y F C o m p O b j b W o r d D o c u m e n t - O b j e c t P o o l / 0 1 2 3 4 5 6 7 8 9 F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; 95-05670 R. Kelly Dawe This is a cytogenetic study of meiosis, or gamete production, in maize, focusing on the structure, the kinetochore, where the meiotic spindle attaches to the chromosome. In the post-doctoral work which serves as a basis for this proposal, image processing of fluorescent chromosomes using deconvolution software now comercially available resulted in more accurate timing of when chromosome pairing occurs during meiosis and a new cytological map for maize chromosome at the start of meiosis. Using mutant maize inbred lines, evidence was obtained to support the hypothesis that the kinetichore is a prerequisite to spindle formation in meiosis and that kinetochore-like structures appear in an inbred line with an abnormal chromosome 10. Dr Dawe will now pursue higher resolution imaging in order to achieve 3D reconstruction of the kinetochore itself. Antibodies which have been used to fluorescently tag the kinetochore will now be used to map its substructure. The proteins which code for these kinetochore proteins will be cloned and monoclonal antibodies prepared. These reagent s and select inbred lines will be used to map the structural differences between the meiotic and mitotic kinetochores, between kinetochores involved in reductional vs. equatorial division, and between the normal kinetochore and the kinetochore-like structure found in a line with abnomral chromosome 10. %%% The meiotic kinetochore has historically provided important insights into the broader functions of the kinetochore, one of the most important structures on the chromosome. It has been shown to bind telomeric DNA, hold sister chromatids together, bind to spindle microtubules, interact with molecular motors on the spindle,and produce a signal that is used as a cell cycle check point. Insight into the way it does this will come with this study which dissects the components of the kinetochore and shows how they interact and change spacially with time. *** ; Oh +' 0 $ H l S u m m a r y I n f o r m a t i o n ( . D h R:\WWUSER\TEMPLATE\NORMAL.DOT 95-05670 Una Solomon Una Solomon @ O @ @ O @ F # Microsoft Word 6.0 2 ; e 3 e - p p p p p p p u 1 7 W T 3 u p u p p p p p p p p i 95-05670 R. Kelly Dawe This is a cytogenetic study of meiosis, or gamete production, in maize, focusing on the structure, the kinetochore, where the meiotic spindle attaches to the chromosome. In the post-doctoral work which serves as a basis for this proposal, image processing of fluorescent chromosomes using deconvolution software now comercially available resulted in more accurate timing of when chromosome pairing occurs during meiosis and a new cytological map for maize chromosome at the start of meiosis. Using mutant maize inbred lines, evidence was obtai

; r o o o t e n t r y f c o m p o b j b j b j b j b j b j b d d o c u c u c u c c u m e n t -o b j e c t p o l / 0 1 2 3 4 5 6 7 8 9 f Microsoft Word 6.0文档MSWORDDOC Word.document.document.document.6; 6; 95-05670 R. Kelly Dawe，这是对玉米中减数分裂或配子产生的细胞遗传学研究，重点是结构，该结构是染色体上的减数分裂纺锤体附着的结构。 在作为该提案的基础的博士后工作中，现在使用反卷积软件对荧光染色体进行图像处理，现在可销售的荧光染色体导致更准确的时机，何时在减数分裂期间发生染色体配对，以及在减生症最初开始进行玉米染色体的新细胞学图。 使用突变玉米近交系列，获得了证据来支持以下假设：kinetichore是减数分裂中纺锤体形成的先决条件，类似动型的结构出现在近交系列中，并带有异常的染色体10。DaweDr Daw将在较高的分辨率成像中实现3D kineterion的更高分辨率成像。 现在，用于荧光标记动力学的抗体现在将用于绘制其子结构。 这些动力学蛋白代码的蛋白质将被克隆并制备单克隆抗体。 这些试剂S和精选的杂交线将用于绘制减数分裂和有丝分裂动物学之间的结构差异，在赤道分裂与赤道分裂涉及的动力学之间以及正常的动型与动力学的结构之间的结构之间，与近代圆形呈繁殖的量子元素的悠久; Kinetochore，染色体上最重要的结构之一。 已显示它可以将端粒DNA结合，将姐妹染色质固定在一起，与纺锤体微管结合，与主轴上的分子电动机相互作用，并产生一个被用作细胞周期检查点的信号。洞察这项研究将涉及这项研究，该研究剖析了动力学的组成部分，并展示了它们如何随时间进行空间互动和变化。 ***; 哦 +'0 $ h l s u m m a r y i i n f o r m a t i o n（。d h r：\ wwuser \ template \ normal.dorm.dot 95-05670 una solomon una una solomon @ o @ o @ o @ o @ o @ o @ o @ f＃microsoft Word 6.0 2; E 3 E -P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P I 95-05670 R. Kelly Dawe这是一项对玉米的减数分裂或配子生产的细胞遗传学研究，重点是结构，重点放在结构上，并集中在牛皮化学上，在这里，牛皮化型固定在牛皮化合物上。 在作为该提案的基础的博士后工作中，现在使用反卷积软件对荧光染色体进行图像处理，现在可销售的荧光染色体导致更准确的时机，何时在减数分裂期间发生染色体配对，以及在减生症最初开始进行玉米染色体的新细胞学图。 使用突变玉米近交系，证据