The Movement and Geographic Origin of Marine Larvae: A Molecular and Genetic Approach

海洋幼虫的运动和地理起源：分子和遗传学方法

基本信息

批准号：
9203320
负责人：
Thomas Hilbish
金额：
$ 26万
依托单位：
University of South Carolina at Columbia
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1992
资助国家：
美国
起止时间：
1992-06-01 至 1995-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9203320&HistoricalAwards=false
关键词：
Movement Geographic Origin Marine Larvae

项目摘要

In order to understand the role of recruitment in the population dynamics of marine species which have high larval dispersal it is necessary to understand the movement of the larvae, their geographic origin and the forces that control their local abundance. Methods for tracking larval movements have been restricted to short time scales or within closed systems but their application to high dispersal systems has been of limited value because of the difficulties with larval recovery. The mussel Mytilus edulis consists of a complex of sibling species that hybridize in discrete areas of contact between the parental forms. Individual mussel larvae can be genetically identified as having originated from either of the parental population, or from populations that contain hybrids. Hybrid larvae can only have originated from populations that contained both parent taxa or F1 hybrids. Using this system it will be possible to obtain information on the origin of larvae at several geographic scales. New techniques are required to genetically identify individual larvae. The methods which will be developed in this research program will allow individual larvae to be assayed directly, with minimal preparation and a high rate of processing. Once the techniques have been developed it will be possible to identify the geographic origin of dispersing or recruited larvae. Once developed this new technique will be employed to study larval dispersal in regions of contact between Mytilus populations.

为了了解幼虫扩散程度高的海洋物种种群动态中招募的作用，有必要了解幼虫的运动、其地理起源以及控制其当地丰度的力量。跟踪幼虫运动的方法仅限于短时间尺度或封闭系统内，但由于幼虫恢复困难，它们在高扩散系统中的应用价值有限。贻贝由一系列同胞物种组成，这些物种在亲本之间的离散接触区域杂交。个体贻贝幼虫可以从基因上鉴定为源自亲代种群或含有杂交种群的种群。杂交幼虫只能起源于同时包含亲本类群或 F1 杂交种的种群。使用该系统将有可能获得有关多个地理尺度的幼虫起源的信息。需要新技术来对个体幼虫进行基因鉴定。该研究计划将开发的方法将允许直接对个体幼虫进行分析，只需最少的准备工作和高处理率。一旦技术得到发展，就有可能确定分散或招募幼虫的地理起源。一旦开发出来，这项新技术将用于研究贻贝种群之间接触区域的幼虫扩散。