Acquisition of a 9.4 Tesla Magnet for Solid State NMR

获取用于固态 NMR 的 9.4 特斯拉磁铁

• 批准号: 8820750
• 负责人: Timothy Cross
• 金额: $ 13.7万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820750&HistoricalAwards=false
• 关键词: Acquisition; 9.4; Tesla; Magnet; Solid

Acquisition of a wide bore 9.4 Tesla magnet for the assembly of a solid state NMR spectrometer is proposed. This research is directed toward the structural and dynamic characterization of protein complexes (collagen), protein lipid complexes (Gramicidin A in a lipid bilayer) and DNA liquid crystalline phases. Further use of the instrument will be made by individuals in a wide range of disciplines as time permits. Solid state NMR is beginning to play a major role in biophysics, especially in the realm of dynamics. The other approaches to dynamic characterizations do not yield the same sort of detail that is possible with solid state NMR. It is possible from specific site labeled biomolecules to determine a model for molecular motions with atomic resolution. This model includes a description of the axis about which the motion is occurring, the type of motion, whether it be diffusional or discontinuous, the amplitude of the motion and finally the frequency of the motion. With such characterizations of the dynamics the potential for obtaining correlations between dynamics and function is very high. Many of the specific experiments described herein would not be possible on the present relatively low field (4.7 Tesla) solid state NMR instrumentation in the FSU facility, not only because of the low field but also because of the severely limited time available on it. A detailed justification for a high field (9.4 Tesla) instrument is given, based on improved sensitivity (Boltzman distribution), improved resolution (by minimizing field dependent 14 N line broadening effects on the 13 C spectra), observation of low gyromagnetic ratio nuclei, reduced acoustic ringing (for 2 H spectra), and observation of quadrupolar nuclei with nonintegral spin.

建议采购宽口径 9.4 特斯拉磁体，用于组装固态核磁共振波谱仪。 本研究针对蛋白质复合物（胶原）、蛋白质脂质复合物（脂质双层中的短杆菌肽 A）和 DNA 液晶相的结构和动态表征。 如果时间允许，各个学科的个人将进一步使用该工具。 固态核磁共振开始在生物物理学中发挥重要作用，特别是在动力学领域。 其他动态表征方法无法提供与固态 NMR 相同的细节。 可以从标记的生物分子的特定位点确定具有原子分辨率的分子运动模型。 该模型包括对发生运动的轴的描述、运动的类型（无论是扩散的还是不连续的）、运动的幅度以及最后的运动频率。 通过这种动力学表征，获得动力学和函数之间的相关性的潜力非常高。 本文描述的许多具体实验在 FSU 设施中目前相对低场（4.7 特斯拉）的固态 NMR 仪器上是不可能的，不仅因为低场，而且因为其可用时间严重有限。 给出了高场（9.4特斯拉）仪器的详细理由，基于改进的灵敏度（玻尔兹曼分布）、改进的分辨率（通过最大限度地减少对 13 C 光谱的场相关 14 N 线展宽效应）、低旋磁比核的观察、减少声振铃（对于 2 H 谱），以及观察具有非整数自旋的四极核。