Graphene quantum dots-based electrochemiluminescence detection of DNA using multiple cycling amplification strategy

Graphene quantum dots-based electrochemiluminescence detection of DNA using multiple cycling amplification strategy

In this study, a novel strategy for amplified electrochemiluminescence (ECL) detection of DNA was proposed based on excellent ECL activity of graphene quantum dots (GQDs) coupled with multiple cycling amplification technique. A new type of graphene QDs with well ECL property and uniform size were firstly synthesized, then the graphene QDs were assembled on the electrode by poly (diallyldimethylammonium chloride) (PDDA)-graphene oxide (GO) nanocomposites, which could greatly improve ECL signal and stability of QDs. A novel signal on ECL biosensor for DNA analysis was designed by using ECL quenching of gold nanoparticles (NPs) to graphene QDs combined with endonuclease-aided cyclic amplification strategy. As a result, the proposed strategy can be conveniently expanded to other biosensing application, especially in clinical diagnoses.

在这项研究中，基于石墨烯量子点（GQDs）优异的电化学发光（ECL）活性并结合多重循环放大技术，提出了一种用于DNA的放大电化学发光检测的新策略。首先合成了一种具有良好电化学发光性能且尺寸均匀的新型石墨烯量子点，然后通过聚二烯丙基二甲基氯化铵（PDDA） - 氧化石墨烯（GO）纳米复合材料将石墨烯量子点组装在电极上，这可以极大地提高量子点的电化学发光信号和稳定性。通过利用金纳米粒子（NPs）对石墨烯量子点的电化学发光猝灭并结合内切酶辅助的循环放大策略，设计了一种用于DNA分析的新型电化学发光生物传感器信号。因此，所提出的策略可以方便地扩展到其他生物传感应用，特别是在临床诊断中。