Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats.

Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats.

UNLABELLED To investigate the effects of Ulinastatin (UTI) in cerebral ischemia-reperfusion (IR) injury in rats and whether this effect might be related to Aquaporin 4 (AQP4), one hundred and eighty adult male Sprague Dawley (SD) rats, weighing 230-280 g, were randomly divided into 3 groups: sham (S) group, IR group and UTI (U) group. Every group was further divided into 3 sub-groups: 6 h group, 24 h group and 48 h group. The transient focal IR injury was induced by inserting a silicone-coater monofilament nylon suture (0.28 mm) from the right external carotid artery to the origin of the left middle cerebral artery. The suture was removed after 2 h to allow reperfusion. UTI treatment group was injected with UTI 100000 u/kg at the beginning of the reperfusion period, while S group and IR group were injected with the same volume of saline. Samples were taken according to the reperfusion time (6 h, 12 h and 24 h). Infract volume was measured by triphenyl tetrazolium chloride staining, and brain water content was determined by wet-dry weight method and neurological scores were checked with a five-point scale. Expression levels of AQP4 were checked with immunohistochemistry and Western blot. RESULTS Compared with S group, the infarct volume, water content, neurological scores and AQP4 levels in the rat brain tissues were significantly increased in IR group. Administration of UTI significantly decreased the infarct volume, water content of the brain tissue and neurological scores. Moreover, the expression levels of AQP4 were also down-regulated by UTI treatment. CONCLUSION UTI improves cerebral IR injury in rats potentially via decreasing the expression levels of AQP4.

无标签 为研究乌司他丁（UTI）对大鼠脑缺血再灌注（IR）损伤的影响以及这种影响是否可能与水通道蛋白4（AQP4）有关，180只体重230 - 280 g的成年雄性斯普拉格 - 道利（SD）大鼠被随机分为3组：假手术（S）组、IR组和UTI（U）组。每组又进一步分为3个亚组：6 h组、24 h组和48 h组。通过将一根硅涂层单丝尼龙缝线（0.28 mm）从右侧颈外动脉插入到左侧大脑中动脉起始处来诱导短暂局灶性IR损伤。2 h后取出缝线以实现再灌注。UTI治疗组在再灌注开始时注射UTI 10,000 U/kg，而S组和IR组注射相同体积的生理盐水。根据再灌注时间（6 h、12 h和24 h）取材。采用氯化三苯基四氮唑染色测量梗死体积，干湿重法测定脑含水量，并采用五分制量表检查神经功能评分。采用免疫组织化学和蛋白质印迹法检测AQP4的表达水平。 结果 与S组相比，IR组大鼠脑组织的梗死体积、含水量、神经功能评分和AQP4水平均显著升高。给予UTI可显著降低梗死体积、脑组织含水量和神经功能评分。此外，UTI治疗也下调了AQP4的表达水平。 结论 UTI可能通过降低AQP4的表达水平改善大鼠脑IR损伤。