Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds

Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds

Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10 days in consistent dosages averaging 80.5 +/- 12.5 (SD) ng/mL. Moreover, the BDNP released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

接种表达脑源性神经营养因子（BDNF）细胞的琼脂糖神经引导支架（NGS）已在大鼠中枢神经系统中显示出强大的神经再生能力。这项工作的目的是探索用氢键逐层涂层（HLbL）的琼脂糖NGS是否能提供一种无细胞的方法来递送持久且剂量一致的活性BDNF。我们的结果表明，HLbL涂层的琼脂糖NGS可在10天内以平均80.5±12.5（标准差）纳克/毫升的一致剂量释放BDNF。此外，通过体外细胞增殖试验证实从HLbL释放的BDNF具有活性。据我们所知，这是首次报道表明组装在水凝胶上的HLbL能够以临床相关剂量持续、长时间地释放活性BDNF。（C）2015 Acta Materialia Inc.，由Elsevier Ltd.出版。保留所有权利。