载脂蛋白M基因敲除小鼠模型的构建和鉴定

载脂蛋白M基因敲除小鼠模型的构建和鉴定

Human apolipoprotein M (ApoM) was discovered by Xu et al [1] in 1999 and mainly exists in high-density lipoprotein (HDL) with anti-atherosclerotic effects [2]. Studies have shown that leptin, insulin, hyperglycemia and various cytokines can regulate the expression of ApoM [3 - 4], and it may also be related to the occurrence and development of obesity, diabetes, liver cancer and colon cancer [5 - 6], but its mechanism of action is not yet clear and its function needs further research. The purpose of this study is to construct an ApoM gene knockout mouse model and establish a method for genotyping ApoM gene knockout mice.

人载脂蛋白M(ApoM)由Xu等[1]于1999年发现,主要存在于具有抗动脉粥样硬化作用的高密度脂蛋白(HDL)中[2].研究显示,瘦素、胰岛素、高血糖以及多种细胞因子可调节ApoM的表达[3-4],也可能与肥胖、糖尿病、肝癌、结肠癌的发生发展相关[5-6],但其作用机制尚不明确,其功能有待进一步研究.本研究旨在构建ApoM基因敲除小鼠模型并建立分型ApoM基因敲除小鼠的方法。